Toward early glaucoma diagnostics: the use of nonlinear optical imaging techniques
Speaker:Dr. Tim Lei
Department of Electrical Engineering, Bioengineering
Physiology and Medicine
University of Colorado, Denver (USA)
Date & Time:31 May 2012 (Thursday) 11:15 - 12:20
Organized by:Department of Electrical and Computer Engineering


Glaucoma has been the leading cause of blindness in the United States and affects 60 million people worldwide. One of the major risk factors of Glaucoma is the evaluation pressure of the intraocular fluid. The increased fluid pressure in the eye is believed to cause damages to the optical nerves and ultimately results in permanent loss of vision. Therefore, it is important to develop non-invasive optical imaging techniques to identify early degeneracy in the fluid outflow system inside the eye to prevent the fluid pressure build up. Nonlinear optical imaging techniques allow functional and molecular specific imaging in biological tissues without the needs of exogenous fluorescent labels. In this talk, I will discuss the technical challenges of imaging the trabecular meshwork, which is the entrance of the outflow system of the eye and our achievements of using nonlinear optical techniques including second harmonic generation, two-photon excitation auto-fluorescence and coherent anti-Stokes Raman scattering microscopy to achieve excellent imaging resolutions and to extract valuable diagnostic information to determine the health of the fluid outflow system in the eye to determine early onsets of glaucoma.


Dr. Tim C. Lei is an assistant professor in the Electrical Engineering Department at the University of Colorado Denver. In addition, he also holds adjunct appointments of the Departments of Bioengineering, Physiology and Biophysics, and Medicine at the University. His research interests focus on applying and developing advanced optical imaging and spectroscopic techniques to study important biomedical problems. Several projects that he is currently researching include: 1) developing nonlinear imaging techniques for early glaucoma detection and diagnostics, 2) measuring protein dynamics and interactions using fluorescent correlation techniques in cell membranes and in lipid rafts for renal diseases, and 3) determining the optical scattering properties of brain tissue to understanding neural circuitry functions using optogenetic proteins. He has published more than dozen of papers on prestigious journals and has been also holding State of Colorado Bioscience Discovery Evaluation Grant since 2009. He has won American Physical Society Travel Award (2002) from American Physical Society & the Outstanding Research and Creative Activity Award in 2008 from University of Colorado Denver.